山羊源副流感病毒3型的分离与分子鉴定

doi:10.11843/j.issn.0366-6964.2015.02.024

Abstract

Abstract:

From Aug 2013 to Mar 2014，fattened goat herds of many farms in Jiangsu province suffered severe respiratory disease.Serum and nasal swab samples were collected and subjected to pathogen detection.Parainfluenza virus type 3 (PIV3) was detected in most of the samples.Positive samples were inoculated onto Madin-Darby bovine kidney (MDBK) cells and passaged 5 times for virus isolation.A novel strain，named JS2013，was successfully isolated as identified by RT-PCR and hemagglutination test.Entire M coding region was amplified and sequenced.Phylogenetic analysis and comparison to other reference sequences available in GenBank indicated JS2013 was clearly distinct from other HPIV3 and BPIV3 strains.This is the first isolation and partial genetic identification of PIV3 from diseased goats.

CLC Number:

S852.659.5

LI Wen-liang，MAO Li，CHENG Su-ping，WANG Qiu-sheng，HUANG Jia-chun，ZHANG Wen-wen，YANG Lei-lei，JIANG Jie-yuan. Isolation and Identification of Caprine Derived Parainfluenza Virus Type 3[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 344-348.

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Isolation and Identification of Caprine Derived Parainfluenza Virus Type 3

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